9WOX

Crystal Structure of the MLH1 Protein Bound to the FAN1 Peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	I 2 2 2
Unit cell lengths	84.795, 138.570, 142.484
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.430 - 3.050
R-factor	0.2331
Rwork	0.230
R-free	0.29040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.025
RMSD bond angle	1.715
Data reduction software	DIALS ((1.20.1_4487: ???))
Phasing software	PHASER
Refinement software	PHENIX (1.21)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.340	3.130
High resolution limit [Å]	3.050	3.050
Number of reflections	16365	1190
<I/σ(I)>	7.3
Completeness [%]	99.8
Redundancy	12.6
CC(1/2)	0.995	0.931

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289	0.2 M Lithium sulfate monohydrate, 0.1 M TRIS hydrochloride, 30% w/v Polyethylene glycol 4,000