9WN0
Crystal structure of phospholipase A2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2022-05-05 |
| Detector | APEX II CCD |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 |
| Unit cell lengths | 86.010, 86.087, 88.570 |
| Unit cell angles | 86.67, 73.37, 86.44 |
Refinement procedure
| Resolution | 44.640 - 2.000 |
| R-factor | 0.2183 |
| Rwork | 0.216 |
| R-free | 0.26840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.028 |
| Data reduction software | HKL-3000 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_3885: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.760 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 148596 | 7411 |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 89.7 | |
| Redundancy | 1.1 | |
| CC(1/2) | 0.970 | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 15% PEG1500,0.1M mes 6.0 |
