9WLV
The Crystal Structure of Alpha-Beta-fold_hydrolase from Microlunatus sagamiharensis.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-08-12 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.953729 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 125.212, 125.212, 79.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.288 - 1.900 |
| Rwork | 0.144 |
| R-free | 0.17940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.636 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.288 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.131 | 0.986 |
| Number of reflections | 50439 | 3188 |
| <I/σ(I)> | 21.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 25.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Bis-Tris pH6.1, 1.8M (NH4)2SO4 + 0.25% 1,3,5-Pentanetricarboxylic acid, 0.25% 4-Hydroxyphenylacetic acid, 0.25% Benzoic acid, 0.25% Poly(3-hydroxybutyric acid),0.02 M HEPES Na pH6.8 |
