9WLP
Crystal structure of monkeypox virus A30/H2 sub-complex at pH 6.8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-22 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 60.410, 116.210, 125.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.220 - 2.500 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.777 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.930 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.124 | 1.154 |
| Number of reflections | 31282 | 3460 |
| <I/σ(I)> | 17.2 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13 | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Potassium nitrate (pH 6.8) and 20% w/v PEG3350 |
