9WK0
Neoantigen Rac1P29S-HLA-A2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-09-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.300, 80.060, 56.620 |
| Unit cell angles | 90.00, 113.18, 90.00 |
Refinement procedure
| Resolution | 25.210 - 1.800 |
| R-factor | 0.1703 |
| Rwork | 0.169 |
| R-free | 0.21450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.910 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.900 | 2.110 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 40393 | 1 |
| <I/σ(I)> | 16.2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.6 | |
| CC(1/2) | 0.999 | 0.782 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M Sodium Chloride, 22% PEG 3000,0.1M HEPES,pH 7.5 |
