9WIU
SbSOMT in apo state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.393, 160.067, 200.964 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.162 - 2.040 |
| Rwork | 0.227 |
| R-free | 0.24690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.505 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.126)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 200.960 | 200.960 | 2.070 |
| High resolution limit [Å] | 2.040 | 11.170 | 2.040 |
| Rmerge | 0.248 | 0.062 | 7.020 |
| Rmeas | 0.269 | 0.069 | 7.703 |
| Rpim | 0.103 | 0.029 | 3.130 |
| Number of reflections | 172235 | 1206 | 8501 |
| <I/σ(I)> | 6.8 | 15.2 | 1 |
| Completeness [%] | 99.3 | 98.8 | 100 |
| Redundancy | 13.2 | 9.1 | 11.3 |
| CC(1/2) | 0.995 | 0.992 | 0.389 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Sodium Malonate pH 7.0, 20% PEG 3350, 10% Glycerol |
