9WHD
Crystal Structure of a GH167 Enzyme from Wenyingzhuangia aestuarii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-06-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.623, 113.341, 241.804 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.440 - 2.000 |
| R-factor | 0.2183 |
| Rwork | 0.217 |
| R-free | 0.26940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.057 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1.19.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.480 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.150 | |
| Number of reflections | 109527 | 5269 |
| <I/σ(I)> | 14.1 | |
| Completeness [%] | 99.4 | 97.9 |
| Redundancy | 12.7 | 12.4 |
| CC(1/2) | 0.997 | 0.732 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.1 M MES pH 6.5, 12% (w/v) PEG 20000 |
