9WHA

Crystal Structure of Bovine STING bound to 2'3'-cGAMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-12
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	50.729, 57.910, 132.269
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.050 - 1.600
R-factor	0.2172
Rwork	0.216
R-free	0.24130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.687
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.130	1.630
High resolution limit [Å]	1.600	1.600
Number of reflections	52228	2542
<I/σ(I)>	7.5
Completeness [%]	99.8
Redundancy	11.2
CC(1/2)	0.991	0.953

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1M Sodium acetate trihydrate pH4.6, 2M Sodium chloride