9WH7

Crystal Structure of Duck STING bound to 2'3'-cGAMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-12
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 65 2 2
Unit cell lengths	83.535, 83.535, 353.159
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.680 - 2.490
R-factor	0.1962
Rwork	0.194
R-free	0.23140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.525
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.290	2.600
High resolution limit [Å]	2.490	2.490
Number of reflections	26681	2860
<I/σ(I)>	13.5
Completeness [%]	99.7
Redundancy	30.1
CC(1/2)	0.998	0.938

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	1 M Ammonium sulfate, 0.1 M Bis-Tris pH5.5, 1% polyethylene glycol 3350