9WE5
UDP-Glucose-bound UgtP with deletion of Pro48-Met91 and insertion of a Ser-Ser-Ser linker
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-22 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.018 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 131.120, 64.080, 110.660 |
| Unit cell angles | 90.00, 107.23, 90.00 |
Refinement procedure
| Resolution | 48.045 - 3.700 |
| Rwork | 0.230 |
| R-free | 0.31050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.671 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.900 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmeas | 0.245 | 1.153 |
| Number of reflections | 9535 | 1357 |
| <I/σ(I)> | 5.49 | 1.45 |
| Completeness [%] | 99.4 | 98.7 |
| Redundancy | 3.6 | 3.55 |
| CC(1/2) | 0.989 | 0.612 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1 M MES, 5% (w/v) PEG6000 |
