9WAS

Human mitochondrial ClpP in complex with LZL25

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	193.15
Detector technology	PIXEL
Collection date	2024-12-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9786
Spacegroup name	C 1 2 1
Unit cell lengths	143.173, 151.847, 104.553
Unit cell angles	90.00, 118.46, 90.00

Refinement procedure

Resolution	83.779 - 3.519
R-factor	0.1837
Rwork	0.178
R-free	0.24720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.884
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.779	3.710
High resolution limit [Å]	3.519	3.520
Number of reflections	23841	20553
<I/σ(I)>	6.9
Completeness [%]	97.7
Redundancy	2
CC(1/2)	0.981	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1M sodium acetate trihydrate pH4.6 8% w/v Polyethylene Glycol 4000