9W8X
Structure of yeast Pol II complexed with a longer scaffold containing a cisplatin-induced inter-strand crosslink lesion at the +5 position.
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.86 |
| Software | cryoSPARC,PHENIX |
| CTF correction | NONE |
