9W7A

Crystal structure of L-galactose dehydrogenase from Luteolibacter sp. strain LG18 in complex with L-glucose and NADP+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2021-11-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	79.329, 89.954, 96.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.017 - 1.560
Rwork	0.160
R-free	0.18380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.496
Data reduction software	XDS
Data scaling software	pointless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0431)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.100	1.590
High resolution limit [Å]	1.560	1.560
Number of reflections	98431	4808
<I/σ(I)>	21.1
Completeness [%]	100.0
Redundancy	6.1
CC(1/2)	0.999	0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	30%(w/v) PEG 8000 200 mM Ammonium sulfate