9W5W
Crystal structure of Namat in complex with NAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-12-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.039, 104.525, 119.763 |
| Unit cell angles | 109.25, 100.66, 90.36 |
Refinement procedure
| Resolution | 49.210 - 2.300 |
| R-factor | 0.1874 |
| Rwork | 0.185 |
| R-free | 0.22890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.740 |
| Data reduction software | autoPX |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.210 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.109 | 0.402 |
| Rmeas | 0.135 | 0.496 |
| Rpim | 0.078 | 0.287 |
| Total number of observations | 301867 | 15650 |
| Number of reflections | 115304 | 5897 |
| <I/σ(I)> | 6.7 | 2.9 |
| Completeness [%] | 90.3 | |
| Redundancy | 2.6 | 2.7 |
| CC(1/2) | 0.990 | 0.753 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Sodium chloride, 0.1 M HEPES pH 7.5, 1.6 M Ammonium sulfate |
