9W58
Structure of L-glutamate oxidase E617K mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.800, 128.900, 150.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.080 - 1.850 |
| R-factor | 0.1543 |
| Rwork | 0.153 |
| R-free | 0.18710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e1m |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.855 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.390 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 115098 | 5646 |
| <I/σ(I)> | 12.6 | 4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.8 | 8.9 |
| CC(1/2) | 0.997 | 0.916 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 277 | 0.1 M CAPS (pH 10.5), 20% (w/v) PEG8000, and 0.02 M NaCl |
