9W57
Structure of L-glutamate oxidase E617F mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 81.630, 150.260, 150.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.630 - 3.270 |
| R-factor | 0.1718 |
| Rwork | 0.169 |
| R-free | 0.23590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e1m |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.531 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.630 | 3.470 |
| High resolution limit [Å] | 3.270 | 3.270 |
| Number of reflections | 28090 | 4484 |
| <I/σ(I)> | 7.5 | 4.5 |
| Completeness [%] | 96.9 | 97.1 |
| Redundancy | 4.9 | 4.4 |
| CC(1/2) | 0.946 | 0.879 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 20% (w/v) PEG3350, and 0.02 M ZnCl2 |
