9W54

Structure of L-glutamate oxidase in complex with L-glutamate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 61 2 2
Unit cell lengths	124.310, 124.310, 168.801
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	62.150 - 2.550
R-factor	0.1962
Rwork	0.192
R-free	0.24650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2e1m
RMSD bond length	0.003
RMSD bond angle	0.596
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.160	2.660
High resolution limit [Å]	2.550	2.550
Number of reflections	25482	3022
<I/σ(I)>	8.2	2.2
Completeness [%]	99.2	98.9
Redundancy	3.8	3.9
CC(1/2)	0.967	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M CAPS pH10, 1.2 M NaH2PO4, 0.8 M K2HPO4, 0.2 M Li2SO4