9VY9

Structure of MIF binding with Gadolinium ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-30
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979176
Spacegroup name	P 21 21 21
Unit cell lengths	57.804, 101.146, 115.403
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.680 - 1.410
R-factor	0.151
Rwork	0.150
R-free	0.16610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.495
Data reduction software	autoPROC
Data scaling software	Aimless (0.7.4)
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	76.070	76.070	1.490
High resolution limit [Å]	1.410	4.210	1.410
Rmerge	0.177	0.080
Rmeas	0.187
Rpim	0.059	0.031	0.832
Number of reflections	129245	4428	18565
<I/σ(I)>	8.7
Completeness [%]	99.7
Redundancy	9.3
CC(1/2)	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.15 M KBr, 30% w/v polyethylene glycol monomethyl ether 2,000, 10 mM sarcosine