9VY6

Structure of MIF binding with Holmium ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-12-28
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979176
Spacegroup name	C 1 2 1
Unit cell lengths	200.033, 64.491, 92.945
Unit cell angles	90.00, 114.64, 90.00

Refinement procedure

Resolution	80.970 - 3.050
R-factor	0.2219
Rwork	0.219
R-free	0.26780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.609
Data reduction software	autoPROC
Data scaling software	Aimless (0.7.4)
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.910	3.220
High resolution limit [Å]	2.970	2.970
Rmerge	0.190	1.298
Rmeas	0.214
Rpim	0.097	0.691
Number of reflections	16753	838
<I/σ(I)>	5.6	1.6
Completeness [%]	91.2
Redundancy	5
CC(1/2)	0.919	0.516

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M ammonium sulfate, 0.1 M MES pH 6.5, 20% w/v Polyethylene glycol 8,000