9VWP

human OSM in complex with Nb3.43

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98
Spacegroup name	I 41
Unit cell lengths	163.985, 163.985, 51.785
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.000 - 3.030
R-factor	0.2436
Rwork	0.240
R-free	0.27490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.585
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.19_4092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.000	3.140
High resolution limit [Å]	3.030	3.030
Number of reflections	13167	934
<I/σ(I)>	14.6
Completeness [%]	96.8
Redundancy	6.5
CC(1/2)	0.996	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M lithium sulfate monohydrate, 0.1M Tris pH 8.5, 25% PEG 3350