9VWO

human OSM in complex with Nb3.30

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	I 4 2 2
Unit cell lengths	113.350, 113.350, 130.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.640 - 2.450
R-factor	0.2145
Rwork	0.209
R-free	0.26490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.034
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.19_4092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.640	2.550
High resolution limit [Å]	2.450	2.450
Number of reflections	30027	3306
<I/σ(I)>	13.4
Completeness [%]	99.4
Redundancy	13.9
CC(1/2)	0.999	0.635

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M ammonium citrate dibasic, 20% PEG 3350.