9VVS
NAD(P)-dependent oxidoreductase from Kutzneria albida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-04-08 |
| Detector | RIGAKU HyPix-3000 |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 117.710, 117.710, 98.987 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.716 - 2.680 |
| Rwork | 0.188 |
| R-free | 0.24770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.351 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MrBUMP |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.716 | 2.770 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.047 | 0.372 |
| Rmeas | 0.191 | |
| Rpim | 0.047 | |
| Number of reflections | 24402 | 1927 |
| <I/σ(I)> | 12.7 | |
| Completeness [%] | 99.1 | 100 |
| Redundancy | 2 | |
| CC(1/2) | 0.996 | 0.970 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 279.15 | 0.05 M Sodium acetate: Acetic acid pH 5.4, 0.8/1.2 M NaH2PO4/K2HPO4 |
