9VVA
UP1 in complex with RNA dinucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2024-05-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.935, 37.967, 38.599 |
| Unit cell angles | 64.38, 67.63, 84.78 |
Refinement procedure
| Resolution | 34.020 - 1.750 |
| R-factor | 0.1695 |
| Rwork | 0.168 |
| R-free | 0.19950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.803 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.045 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.042 | 0.133 |
| Number of reflections | 16734 | 778 |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 95.4 | 91.7 |
| Redundancy | 3.3 | |
| CC(1/2) | 0.997 | 0.970 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.2M potassium formate, 20% w/v PEG 3350, pH 7.3 |
