9VV8

Crystal structure of the second StART-like domain from Arabidopsis C2 and GRAM domain-containing (C2GR) protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	C 2 2 21
Unit cell lengths	67.234, 69.797, 77.364
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.812 - 1.704
Rwork	0.196
R-free	0.23250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.590
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.065
Number of reflections	19927	1774
<I/σ(I)>	28.53
Completeness [%]	98.3
Redundancy	8.2
CC(1/2)	0.996	0.896

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M Bicine & Tris (pH 8.5), 12.5% MPD, 12.5% PEG1000, and 12% PEG3350.