9VV7
Crystal structure of the first StART-like domain of Arabidopsis C2 and GRAM domain-containing (C2GR) protein in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 65 |
| Unit cell lengths | 87.847, 87.847, 122.940 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.813 - 2.097 |
| Rwork | 0.238 |
| R-free | 0.25750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.737 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.097 | 2.100 |
| Rmerge | 0.111 | 1.489 |
| Number of reflections | 31514 | 3137 |
| <I/σ(I)> | 25.52 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.4 | |
| CC(1/2) | 0.997 | 0.623 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M NaAc (pH 4.6) and 1.8 M MgSO4 |
