9VUN

Crystal structure of the DNA/RNA hybrid, D(CGTCTCCAGGCAAC)/R(GUUGCCUGGAGACG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-05-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	46.239, 47.998, 50.876
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.860 - 2.720
R-factor	0.199
Rwork	0.194
R-free	0.24290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.611
Data reduction software	autoPX
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.910	2.870
High resolution limit [Å]	2.720	2.720
Number of reflections	3308	467
<I/σ(I)>	26.1	3.5
Completeness [%]	99.9
Redundancy	11.3
CC(1/2)	0.999	0.951

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.02 M Magnesium chloride hexahydrate, 0.002 M Cobalt(II) chloride hexahydrate 0.05 M HEPES sodium pH 7.5 2.0 M Ammonium sulfate 0.001 M Spermine