9VTR

Target DNA-bound type I-F3 TniQ-Cascade of Vibrio parahaemolyticus in full R-loop state 2

This is a non-PDB format compatible entry.

EM specimen

Instrument

FEI VITROBOT MARK IV

EM Data Collection

Microscope	JEOL CRYO ARM 300
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Energy Filter	In-column Omega Filter
Detector	GATAN K3 BIOCONTINUUM (6k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.89
Software	cryoSPARC,PHENIX,SerialEM,UCSF,CHIMERAX,RELION
Symmetry	C1
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION