9VTK

Structure of the family PL40 Ulvan Lyase Uly1040

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-10
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	48.997, 126.039, 69.737
Unit cell angles	90.00, 97.96, 90.00

Refinement procedure

Resolution	37.350 - 1.740
R-factor	0.1854
Rwork	0.183
R-free	0.22690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.140
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.560	1.790
High resolution limit [Å]	1.740	1.740
Rmerge	0.133	1.363
Number of reflections	84856	6162
<I/σ(I)>	6.8
Completeness [%]	99.1
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	Sodium bromide, Bis Tris Propone (pH8.7), PEG3350