9VP5

Glucose-6-phosphate dehydrogenase from Leishmania donovani

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-11
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.968
Spacegroup name	C 1 2 1
Unit cell lengths	191.327, 97.356, 70.346
Unit cell angles	90.00, 93.83, 90.00

Refinement procedure

Resolution	54.886 - 2.400
Rwork	0.197
R-free	0.25930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.247
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.886	9.600
High resolution limit [Å]	2.400	2.400
Number of reflections	49811	4586
<I/σ(I)>	6.8
Completeness [%]	98.9
Redundancy	4.8	4.9
CC(1/2)	0.978	0.826

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	4% Tacsimate, 12% PEG3350, 4-8% Glycerol