9VOF

Crystal strucrue of HuHF-C2-ALY complex

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	F 4 3 2
Unit cell lengths	183.330, 183.330, 183.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.560 - 1.900
R-factor	0.1734
Rwork	0.171
R-free	0.19380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.835
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.930
High resolution limit [Å]	1.900	5.160	1.900
Rmerge	0.074	0.047	0.229
Rmeas	0.075	0.047	0.232
Rpim	0.013	0.008	0.038
Total number of observations	776907
Number of reflections	21385	1231	1036
<I/σ(I)>	8.5
Completeness [%]	100.0	100	100
Redundancy	36.3	32.2	37.4
CC(1/2)	0.999	0.999	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Bicine MgCl2