9VOD

Crystal strucrue of HuHF-C2-Cur complex 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	F 4 3 2
Unit cell lengths	183.538, 183.538, 183.538
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.570 - 1.920
R-factor	0.1572
Rwork	0.153
R-free	0.19230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.827
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.570	1.990
High resolution limit [Å]	1.920	1.920
Rmerge	0.061
Rmeas	0.062	0.223
Number of reflections	20691	1958
<I/σ(I)>	16.8	10.14
Completeness [%]	99.6
Redundancy	35.2
CC(1/2)	1.000	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Bincine MgCl2