9VMK
Crystal structure of TeGH116 from Thermosynechococcus elongatus with glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-10-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 268.999, 55.094, 181.996 |
| Unit cell angles | 90.00, 131.86, 90.00 |
Refinement procedure
| Resolution | 26.535 - 2.000 |
| Rwork | 0.169 |
| R-free | 0.18940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.854 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 26.535 | 26.520 | 2.030 |
| High resolution limit [Å] | 2.000 | 10.950 | 2.000 |
| Rmerge | 0.198 | 0.026 | 1.031 |
| Rmeas | 0.280 | 0.037 | 1.458 |
| Rpim | 0.198 | 0.026 | 1.031 |
| Number of reflections | 132197 | 808 | 6440 |
| <I/σ(I)> | 5.4 | 14.6 | 1.3 |
| Completeness [%] | 97.9 | 88.5 | 96.4 |
| Redundancy | 3.8 | 3.2 | 3.9 |
| CC(1/2) | 0.976 | 0.998 | 0.434 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 291 | 1M Potassium sodium tartrate tetrahydrate, 0.1M Bis-Tris propane pH 6.8 |
