9VKM
Crystal structure of the Fe(II)/alpha-ketoglutarate dependent dioxygenase MicC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL18U |
| Synchrotron site | NFPSS |
| Beamline | BL18U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-11-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 61.319, 131.163, 132.248 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 93.130 - 2.200 |
| R-factor | 0.168 |
| Rwork | 0.166 |
| R-free | 0.20140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.921 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.130 | 2.230 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.072 | |
| Number of reflections | 59626 | 6970 |
| <I/σ(I)> | 18.9 | 2.5 |
| Completeness [%] | 96.5 | |
| Redundancy | 7.7 | |
| CC(1/2) | 0.451 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 289.15 | MicC: 10.2 mg/ml in 0.15 M NaCl, 0.02 M Tris, pH 8.0 Precipitant: 19% PEG3350, 200 mM sodium citrate tribasic, 100 mM sodium citrate/citric acid, pH 4.0 |
