9VKJ

Salmonella typhimurium effector protein SteA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-04
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	129.141, 45.151, 48.646
Unit cell angles	90.00, 90.45, 90.00

Refinement procedure

Resolution	32.280 - 2.090
R-factor	0.2137
Rwork	0.212
R-free	0.24440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.854
Data reduction software	HKL-3000
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.280	2.140
High resolution limit [Å]	2.090	2.090
Number of reflections	15867	15867
<I/σ(I)>	12.55
Completeness [%]	96.3
Redundancy	4.3
CC(1/2)	0.997	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		300	0.9M Sodium phosphate monobasic/0.6M Potassium phosphate dibasic,0.1M CAPS/Sodium hydroxide pH 10.5,0.1M Lithium sulfate