9VKF

Crystal structure of yeast arginine N-methyltransferase 1,HMT1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	CCD
Collection date	2025-04-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97
Spacegroup name	P 31
Unit cell lengths	84.670, 84.670, 97.720
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.330 - 2.850
R-factor	0.2349
Rwork	0.235
R-free	0.28370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.690
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.330	2.920
High resolution limit [Å]	2.850	2.850
Rmerge	0.268	1.130
Number of reflections	18335	1379
<I/σ(I)>	15.2
Completeness [%]	100.0
Redundancy	10.1
CC(1/2)	0.993	0.811

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289.15	0.15 M DL-Malic acid pH 7.0, 20% w/v Polyethylene glycol 3,350