9VI5

The structure of coproporphyrin ferrochelatase(CpfC)in staphylococcus aureus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	82
Detector technology	CCD
Collection date	2025-04-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97853
Spacegroup name	P 1
Unit cell lengths	51.161, 54.450, 60.434
Unit cell angles	69.73, 84.85, 90.07

Refinement procedure

Resolution	33.669 - 2.185
R-factor	0.2031
Rwork	0.199
R-free	0.26310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.874
Data reduction software	autoPROC
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.669	2.300
High resolution limit [Å]	2.185	2.185
Number of reflections	30544	4395
<I/σ(I)>	8.2
Completeness [%]	96.8
Redundancy	3.5
CC(1/2)	0.996	0.899

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.2 M Calcium chloride dihydrate, 20% (w/v) Polyethylene glycol 3350