9VGK
Ancestral L-tryptophan synthase beta-subunit 1 complex with Trp-PLP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.817, 100.339, 89.656 |
| Unit cell angles | 90.00, 90.34, 90.00 |
Refinement procedure
| Resolution | 42.620 - 2.200 |
| R-factor | 0.18356 |
| Rwork | 0.180 |
| R-free | 0.24083 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.796 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.700 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 71605 | 71605 |
| <I/σ(I)> | 12.5 | |
| Completeness [%] | 98.6 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.998 | 0.765 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 296 | 20% w/v PEG 3350, 200mM ammonium formate |
