9VFY

Crystal structure of Phaeodactylibacter sp. phosphoglucomutase in complex with glucose-6-phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97861
Spacegroup name	C 1 2 1
Unit cell lengths	108.904, 59.206, 99.542
Unit cell angles	90.00, 118.05, 90.00

Refinement procedure

Resolution	48.060 - 1.970
R-factor	0.1766
Rwork	0.174
R-free	0.22220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.833
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.060	2.070
High resolution limit [Å]	1.970	1.970
Number of reflections	37461	5747
<I/σ(I)>	12.2
Completeness [%]	94.0
Redundancy	6.7
CC(1/2)	0.995	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.15 M DL-maleic acid pH 7.0 0.1 M Imidazole pH 6.5 20% v/v polyethylene glycol monomethyl ether 550 3 mM G6P