9VF4
CryoEM structure of phospholipid-independent cyclised RP4 pilus
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 2.74 |
| Software | cryoSPARC,PHENIX,EPU,UCSF ChimeraX,Coot |
| CTF correction | NONE |
