9VE3

The MIDN Catch-IRF4 (V2L) fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2025-04-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 65 2 2
Unit cell lengths	88.327, 88.327, 72.744
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.500 - 2.100
R-factor	0.2268
Rwork	0.223
R-free	0.26310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.878
Data reduction software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.510	2.210
High resolution limit [Å]	2.100	2.100
Number of reflections	10087	880
<I/σ(I)>	40.3
Completeness [%]	98.1
Redundancy	28.3
CC(1/2)	1.000	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	2% (v/v) tacsimate (pH 5.0), 0.1 M sodium citrate, and 16% (w/v) PEG 3,350