9VCE
Crystal structure of Escherichia coli tryptophanyl-tRNA synthetase in complex with 71g
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.176, 79.272, 77.616 |
| Unit cell angles | 90.00, 105.71, 90.00 |
Refinement procedure
| Resolution | 53.880 - 1.820 |
| R-factor | 0.19013 |
| Rwork | 0.189 |
| R-free | 0.21046 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.760 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.880 | 1.920 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.062 | 0.720 |
| Number of reflections | 63719 | 9263 |
| <I/σ(I)> | 17.9 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.15 M Ammonium sulfate, 0.1 M HEPES pH7.5, 25% w/v PEG3,350 |
