9VB8
Crystal structure of human integrin alpha2 I domain in complex with homo-trimeric collagen model peptide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.364, 71.764, 83.861 |
| Unit cell angles | 110.28, 90.09, 112.21 |
Refinement procedure
| Resolution | 48.670 - 1.600 |
| R-factor | 0.1889 |
| Rwork | 0.185 |
| R-free | 0.22230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.061 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.670 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.120 | 0.552 |
| Rmeas | 0.153 | 0.708 |
| Rpim | 0.093 | 0.438 |
| Total number of observations | 346318 | 16776 |
| Number of reflections | 153836 | 7439 |
| <I/σ(I)> | 4.7 | 1.5 |
| Completeness [%] | 96.5 | |
| Redundancy | 2.3 | 2.3 |
| CC(1/2) | 0.978 | 0.508 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2M ammonium acetate, 0.1M sodium acetate buffer pH 4.6, 30% PEG 4000 |
