9V9O
Crystal structure of CDC48A-N domain in complex with PUX5 SHP box motif and UBX domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-03-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.880, 87.110, 95.970 |
| Unit cell angles | 88.59, 86.67, 89.89 |
Refinement procedure
| Resolution | 95.780 - 2.200 |
| R-factor | 0.226 |
| Rwork | 0.224 |
| R-free | 0.26810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.473 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.1_5286: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.780 | 2.481 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Number of reflections | 38170 | 1908 |
| <I/σ(I)> | 7 | 1.35 |
| Completeness [%] | 86.8 | 38.1 |
| Redundancy | 3.6 | 3.75 |
| CC(1/2) | 0.994 | 0.485 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 100mM SPG Buffer pH 5.5 25% (w/v) PEG 1500 |
