9V9A

Crystal structure of the periplasmic domain of CadF from Campylobacter jejuni in complex with glycine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-23
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9793
Spacegroup name	P 1
Unit cell lengths	27.221, 48.699, 49.695
Unit cell angles	109.84, 98.78, 106.21

Refinement procedure

Resolution	26.180 - 1.750
R-factor	0.1716
Rwork	0.170
R-free	0.20350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.786
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.780
High resolution limit [Å]	1.750	1.750
Number of reflections	21017	957
<I/σ(I)>	16.1	2.2
Completeness [%]	95.3	86.1
Redundancy	3.1	2.3
CC(1/2)	0.994	0.887

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	291	PEG 4000, MES