9V79

Structural basis of signal activation and transduction by chitin elicitor receptor kinase 1 in Oryza sativa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-22
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	P 61 2 2
Unit cell lengths	58.213, 58.213, 283.132
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.410 - 2.120
R-factor	0.238
Rwork	0.234
R-free	0.27540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.729
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.410	2.230
High resolution limit [Å]	2.120	2.120
Number of reflections	15326	1988
<I/σ(I)>	5.4
Completeness [%]	89.8
Redundancy	10.4
CC(1/2)	0.949	0.623

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.08 M Calcium acetate hydrate, 0.2% v/v TacsimateTM pH 4.0, 0.01 M Sodium acetate trihydrate pH 4.6, 9.6% w/v Polyethylene glycol 3,350