9V73

Structural basis of signal activation and transduction by chitin elicitor receptor kinase 1 in Oryza sativa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-20
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	P 61 2 2
Unit cell lengths	58.634, 58.634, 281.662
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.970 - 2.270
R-factor	0.24
Rwork	0.235
R-free	0.27980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.601
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.970	2.390
High resolution limit [Å]	2.270	2.270
Number of reflections	14016	1829
<I/σ(I)>	5.9
Completeness [%]	98.2
Redundancy	10.2
CC(1/2)	0.993	0.547

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.06 M Potassium bromide, 0.02 M Ammonium acetate, 0.01 M HEPES pH 7.5, 12% w/v Polyethylene glycol monomethyl ether 2,000, 2.5% w/v Polyethylene glycol 3,350