9V70

Structural basis of signal activation and transduction by chitin elicitor receptor kinase 1 in Oryza sativa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-05
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	P 31 2 1
Unit cell lengths	109.203, 109.203, 120.457
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.016 - 2.820
R-factor	0.2117
Rwork	0.208
R-free	0.24890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.785
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	120.460	2.890
High resolution limit [Å]	2.820	2.820
Number of reflections	20505	1491
<I/σ(I)>	15.1
Completeness [%]	100.0
Redundancy	19.7
CC(1/2)	0.996	0.757

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	5% v/v Polyethylene glycol 200, 0.05 M BIS-TRIS propane pH 9.0, 9% w/v Polyethylene glycol 8,000