9V5J

Light driven CO2 reduction by fluorescent protein mRuby-CO2M1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-02
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	66.999, 95.662, 31.329
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.930 - 1.740
R-factor	0.2186
Rwork	0.218
R-free	0.23675
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.419
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.930	1.790
High resolution limit [Å]	1.740	1.740
Number of reflections	21446	1532
<I/σ(I)>	8.5
Completeness [%]	100.0
Redundancy	12
CC(1/2)	0.998	0.673

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M NH4HCO3, 0.2M (NH4)2SO4, 0.1M Bistris pH6.5, 24%PEG3350