9V5B

Light driven bicarbonate reduction by fluorescent protein mRuby-Carb2M3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-02
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	31.659, 67.162, 95.045
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.680 - 1.750
R-factor	0.22308
Rwork	0.221
R-free	0.26099
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	1.166
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.690	1.800
High resolution limit [Å]	1.750	1.750
Number of reflections	21225	1531
<I/σ(I)>	7.7
Completeness [%]	100.0
Redundancy	12.2
CC(1/2)	0.996	0.711

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	was 0.2M NH4HCO3, 0.1M Bistris pH6.5, 25%PEG3350