9V50
Crystal structure of Guanine-II riboswitch in complex with 8-methylguanine
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.10213 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 38.040, 52.214, 222.061 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.520 - 3.250 |
| R-factor | 0.2408 |
| Rwork | 0.234 |
| R-free | 0.29700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.322 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (PHENIX 1.18.2-3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 222.060 | 3.420 |
| High resolution limit [Å] | 3.230 | 3.230 |
| Rmerge | 0.296 | |
| Number of reflections | 3562 | 579 |
| <I/σ(I)> | 7 | |
| Completeness [%] | 92.1 | |
| Redundancy | 9.5 | |
| CC(1/2) | 0.995 | 0.931 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.08 M sodium chloride, 0.04 M sodium cacodylate trihydrate (pH 7.0), 30% v/v (+/-)-2-methyl-2,4-pentanediol, and 0.012 M spermine tetrahydrochloride |
